Next generation sequencing analysis of exosomal microRNAs: Fusobacterium nucleatum regulates expression profiling of exosomal microRNAs in human colorectal cancer cells
유미라, 김혜정, 강지완, 김윤학, 박혜련,
소속 상세정보
유미라 ( Yu Mi-Ra ) - Pusan National University School of Dentistry Department of Oral Pathology
김혜정 ( Kim Hye-Jung ) - Pusan National University School of Dentistry Periodontal Disease Signaling Network Research Center
강지완 ( Kang Ji-Wan ) - Pusan National University Interdisciplinary Program of Genomic Science
김윤학 ( Kim Yun-Hak ) - Pusan National University School of Medicine Department of Anatomy
박혜련 ( Park Hae-Ryoun ) - Pusan National University School of Dentistry Department of Oral Pathology
Abstract
Colon cancer is one of the most common malignant tumors, but there are still a few validated biomarkers of coloncancer. Exosome-mediated microRNAs (miRNAs) have been recognized as potential biomarkers in cancers, andmiRNAs can regulate a variety of genes. Recently, Fusobacterium nucleatum was discovered in the tissues of humancolon cancer patients. Its role in colon cancer was highlighted. F. nucleatum may contribute to the progression ofcolon cancer through the mechanism of exosome-mediated miRNAs transfer. However, the exosomal miRNAsregulation mechanism by F. nucleatum in colon cancer is not well known. Thus, we performed next-generationsequencing to investigate the overall pattern of exosomal miRNAs expression in the colon cancer cell culturesupernatant. We have confirmed the alterations of various exosomal miRNAs. In addition, to investigate the functionof exosomal miRNAs, a Kyoto Encyclopedia of Genes and Genomes analysis was performed on the target genesof changed miRNAs. Potential target genes were associated with a variety of signaling pathways, and one of thesepathways was related to colorectal cancer. These findings suggested that F. nucleatum can alter exosomal miRNAsreleased from colorectal cancer cells. Furthermore, exosomal miRNAs altered by F. nucleatum could be potentialbiomarkers for the diagnosis and therapy of colon cancer.
키워드
Colonic neoplasms; Fusobacterium nucleatum; MicroRNAs; Exosomes
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